This very long and challenging stage, with several climbs of medium length, has a significant overall vertical elevation. The race starts in Follonica, to cross the northern part of the province of Grosseto up to the Monte Amiata and then Montalcino towards Chiusi. Once in the Umbria region, the route proceeds from Moiano on a slightly less complex route, up to Montefalco to enter the final Trevi circuit to be covered just once. There are four medium difficulty GPMs: Roccastrada, Passo del Lume Spento and Cibottola and Trevi, where the riders reach the finish line.
Very demanding final circuit. The ascent of Trevi is repeated twice, with a first pass of the finish line before starting the circuit that ends the stage. After the first passage, the climb continues for about 300m with gradients of up to 15%. There is a fast descent on the road from the normal roadway up to the old Flaminia and then, after Borgo Trevi, the final 3km that lead to the finish line. The narrow road has gradients over 13-15% and features some short descents before the last 300m that reach more than 15% ascents. The finish line is on 7.5m wide asphalted roadway.